New Protein Stability Analysis Systems Accelerate Biologics Development

The new Isothermal Chemical Denaturation (ICD) Systems from AVIA will help you accelerate your biologics development programs.

The Technology :

  • Until now stability has been measured by heating proteins until they denature. But, what does Tm really tell you about the stability of your product at room or lower temperature? Practically nothing.
  • Only ICD measures how much protein is in the correct native conformation and how much is denatured and prone to aggregate at room temperature or experimental temperature.
  • Better yet, ICD requires only 25-50 µg of protein per test. With so little protein required, you are able to test more conditions earlier leading to better decisions and lower risk of following misleading or highly limited data.

The result : 

  • you obtain more and better stability information sooner and at lower cost.
  • No other company offers the breadth and depth of ICD expertise and products.
  • To learn more about how ICD technology can help you, please contact us for a free consultation or to schedule a webinar or on-site seminar for you and your colleagues.

Protein Construct Stability Evaluation

  • The stability of multiple protein constructs can be rapidly compared. In a single automated experiment, up to 12 different protein constructs can be compared using up to 8 different formulating conditions for each protein. With protein consumption in the range of only 25-50 µg per test, many conditions can be tested early in the development process without consuming large quantities of precious protein.
  • This capability provides an easy and rapid means to assess the relative potential of suitability for development of each molecule. It also provides an early assessment of formulating conditions most suitable for other biophysical and biochemical testing and purification.

Formulations and Process Conditions Screening and Optimization

The Model 2304 ICD Systems is perfectly suited for quickly screening though series of standard buffers and excipients for pre-formulations studies or screening potential purification conditions for their impact on protein stability.


During formulations optimization, groups of pre-prepared formulations can be tested or the instrument can be programmed to automatically prepared formulations for testing. With protein consumption in the range of 25-50 µg per test, many formulation or process conditions can be tested early in the development process without consuming large quantities of precious protein. 

Ligand Binding Studies

ICD shifts allow accurate determination of binding affinities over a wide dynamic range (from picomolar to micromolar). Binding affinities determined by ICD shifts have been proven to correlate well with ITC determined binding affinities. Determining binding affinities with ICD requires far less sample than what is required for ITC and unlike SPR requires very little methods development since there are no chemical linkers systems involved. ICD is truly a label-free and linker-free system.